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KMID : 1098420110190010031
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Korean Journal of Medicinal Crop Science
2011 Volume.19 No. 1 p.31 ~ p.37
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Protective Effect of an Ethanol Extract Mixture of Curcuma longae Radix, Phellinus linteus, and Scutellariae Radix on Oxidative Neuronal Damage
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Kim Ju-Yeon
Kweon Ki-Yeon
Lee Hong-Kyu
Kim Seung-Hwan
Yoo Jae-Kuk
Bae Ki-Hwan
Seong Yeon-Hee
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Abstract
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Previous work demonstrated that an ethanol extract (HS0608) of a mixture of three medicinal plants of Curcuma longae radix, Phellinus linteus, and Scutellariae radix markedly inhibits Aβ (25-35)-induced neurotoxicity. The present study was performed to further verify the neuroprotective effect of HS0608 on oxidative and ischemic cerebral injury using cultured rat cortical neurons and rats. Exposure of cultured cortical neurons to 100 μM hydrogen peroxide (H2O2) induced neuronal apoptotic death. At 10-100㎍/㎖, HS0608 inhibited neuronal death, elevation of intracellular calcium concentration ([Ca2+]i), and generation of reactive oxygen species (ROS) induced by H2O2 in primary cultures of rat cortical neurons. In vivo, HS0608 prevented cerebral ischemic injury induced by 2-h middle cerebral artery occlusion (MCAO) and 24-h reperfusion. The ischemic infarct and edema were significantly reduced in rats that received HS0608 (200 ㎎/㎏). These results suggest that the anti-oxidative properties of HS0608 may be responsible for its neuroprotective effect against focal cerebral ischemic injury and that HS0608 may have a therapeutic role in neurodegenerative diseases such as stroke.
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KEYWORD
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Curcuma longae Radix, Phellinus linteus, Scutellariae Radix, Neuroprotection, H2O2, Cultured Cortical Neurons, Cerebral Ischemic Injury
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